Affinity chromatography resins

Affinity chromatography resins

Protein A resins that offer high capacity and selectivity

WorkBeads™ affimAb is an optimized alkali-stable protein A resin designed for purification of monoclonal- and polyclonal antibodies in laboratory to process scale. This resin has a superior base matrix combined with a patented, alkali-stable protein A ligand, which results in high dynamic binding capacity at short residence times. This resin binds over 40 mg IgG/mL resin also at as short residence time as 2.5 min. Available also in prepacked 1 mL and 5 mL GoBio™ Mini columns.

• Top performance dynamic binding capacity also at short residence times
• Outstanding alkali stability with 0.5 M NaOH, extends the number of purification cycles
• Excellent purity, recovery and reproducibility
• Negligible protein A leakage

Today, monoclonal antibodies (mAbs) represent the largest and most progressive biopharmaceutical market. The mAbs used for clinical applications are subject to very high purity requirements, and there is, thus, a demand for more efficient and economical purification processes. WorkBeads affimAb allows mAb purification that gives exceptionally low host cell protein (HCP) and DNA (HCD) levels. This high-capacity resin allows a higher purity of eluted mAbs from cell supernatants than the market-leading resin.

Principle of affinity chromatography

Affinity chromatography is a useful technique for the separation of proteins by exploiting the reversible interaction between the target protein and the immobilized ligand. The interaction can be bio-specific, for example, antibodies binding to protein A, or not bio-specific, such as histidine-tagged proteins binding to metal ions.

Affinity chromatography is a widely used technique for purifying antibodies due to its high selectivity, resolution, and capacity. High purity is often achieved in a single step. Large sample volumes can be processed, and samples can be applied under conditions that favour specific binding to the ligand. Elution is often carried out under gentle conditions, which helps preserve bioactivity. The target protein is eluted in a purified and concentrated form by modification of pH and ionic strength or by introducing a competitive agent.

WorkBeads give unbeatable results for biomolecule purification

WorkBeads are agarose-based chromatographic resins manufactured using a proprietary method that results in porous beads with tight size distribution and exceptional mechanical stability. Agarose-based matrices have been successfully used for decades in biotechnology purification, from research to production scale, due to their exceptional compatibility with biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. WorkBeads resins are designed for separations requiring optimal capacity and purity. 

Don't hesitate to contact us about receiving a sample resin to ensure purification success on your target molecule.