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Trends and know-how in biotech and downstream purification.

The challenge of phosphorothioate oligonucleotidepurifications - AIEX

Introduction Since oligonucleotides (ONs) are mainly synthetically produced by solid-phase synthesis it is easy to modify them during the synthesis process. The most commonly used modification,...

Effects of elution conditions in the purification of oligonucleotides

Introduction Oligonucleotide synthesis provides custom-made oligonucleotides. The purity of oligonucleotide preparations produced by solid-phase synthesis can be very high. However, purification is...

Conversion of ion-pairing agents/counter ions for oligonucleotides using AIEX

Introduction Efficient purification of therapeutic molecules is of the utmost importance. Equally important, is the removal of residuals, such as ion-pairing agents (IP agents), used in the...

Important optimization parameters for AIEX purifications of 45 nt-long RNA molecules

Introduction RNA molecules have emerged as important molecules in therapeutics, primarily in the form of antisense oligos (ASO) and small interfering RNAs (siRNAs). Consequentially, requirements for...

Optimization and Scale-up of Oligonucleotide Purifications

Introduction Solid-phase synthesis of oligonucleotides generally gives material of rather high purity. However, for many applications, and especially for therapeutics, there is a need for...

Addressing the challenge of optimizing His-tagged protein purifications

Introduction Immobilized Metal Affinity Chromatography (IMAC) is the commonest technique for purification of His-tagged proteins. Tagging proteins enables the use of affinity chromatography for...

Easy optimization of His-tagged protein purification

Introduction Immobilized Metal Ion Affinity Chromatography (IMAC) utilizes the affinity of histidine, cysteine and tryptophan on the protein surface for binding to metal ions immobilized on the...

Improved key quality attributes of antibody purification processes

Introduction The rapidly growing monoclonal antibody (mAb) market and the increasing demand for improved process economy put pressure on creating efficient downstream processing. WorkBeads™ affimAb...

Optimized purification of antibodies using a new high-capacity and alkaline stable protein A resin

Introduction A new protein A chromatography resin has been designed for purification of mAbs, Gabs and polyclonal antibodies. The new resin, WorkBeads™ affimAb, is designed for high dynamic binding...

Efficient protection of protein A resins during mAb purifications

Introduction The purity requirements are high for monoclonal antibodies (mAbs). Using protein A chromatography resins result in mAbs with high yield and purity in a single step and is therefore the...