Blog

Trends and know-how in biotech and downstream purification.

Addressing the challenge of optimizing His-tagged protein purifications

Introduction Immobilized Metal Affinity Chromatography (IMAC) is the commonest technique for purification of His-tagged proteins. Tagging proteins enables the use of affinity chromatography for...

Easy optimization of His-tagged protein purification

Introduction Immobilized Metal Ion Affinity Chromatography (IMAC) utilizes the affinity of histidine, cysteine and tryptophan on the protein surface for binding to metal ions immobilized on the...

Improved key quality attributes of antibody purification processes

Introduction The rapidly growing monoclonal antibody (mAb) market and the increasing demand for improved process economy put pressure on creating efficient downstream processing. WorkBeads™ affimAb...

Optimized purification of antibodies using a new high-capacity and alkaline stable protein A resin

Introduction A new protein A chromatography resin has been designed for purification of mAbs, Gabs and polyclonal antibodies. The new resin, WorkBeads™ affimAb, is designed for high dynamic binding...

Efficient protection of protein A resins during mAb purifications

Introduction The purity requirements are high for monoclonal antibodies (mAbs). Using protein A chromatography resins result in mAbs with high yield and purity in a single step and is therefore the...

A novel protein A-based resin for optimized purification of antibodies

Introduction A protein A ligand designed to give high alkaline stability was immobilized on WorkBeads™ basematrix. The combination of ligand concentration and resin properties was optimized for high...