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Trends and know-how in biotech and downstream purification.

Desalting of antisense oligonucleotides and other oligonucleotides

Oligonucleotides as therapeutic molecules Short oligonucleotides have become established as important molecules in therapeutics, primarily in the form of antisense oligonucleotides (ASOs), aptamers...

AIEX purification parameters for phosphorothioate oligonucleotides

Introduction Oligonucleotides with a phosphorothioate backbone Oligonucleotide (ON)-based therapeutics is an evergrowing market with an increasing number of both FDA approved ON drugs and clinical...

Properties of WorkBeads 40 IEX resins: What makes them exceptional?

Chromatography agarose resins Agarose-based chromatography resins consisting of highly cross-linked porous beads have been used for decades to purify and separate a diverse set of macromolecules....

The challenge of phosphorothioate oligonucleotidepurifications - AIEX

Introduction Since oligonucleotides (ONs) are mainly synthetically produced by solid-phase synthesis it is easy to modify them during the synthesis process. The most commonly used modification,...

What is: Ion Exchange Chromatography (IEX) ?

Introduction Ion Exchange Chromatography (IEX) is a powerful and widely used technique for separating and purifying biomolecules such as proteins, peptides, and oligonucleotides. This technique is...

Effects of elution conditions in the purification of oligonucleotides

Introduction Oligonucleotide synthesis provides custom-made oligonucleotides. The purity of oligonucleotide preparations produced by solid-phase synthesis can be very high. However, purification is...

Purification of oligonucleotides by anion exchange chromatography

Solid-phase synthesis of oligonucleotides generally give material of rather high purity. However, for many applications, and especially for therapeutics, there is a need for purification to remove...

Conversion of ion-pairing agents/counter ions for oligonucleotides using AIEX

Introduction Efficient purification of therapeutic molecules is of the utmost importance. Equally important, is the removal of residuals, such as ion-pairing agents (IP agents), used in the...

A comparative study of ion exchange vs. ion pair chromatography for preparative separation of oligonucleotides (2025)

The Study: A Comparative Analysis Our study, published in the Journal of Chromatography A, focused on the preparative purification of a 20-mer oligonucleotide. We systematically compared IEX and...

Important optimization parameters for AIEX purifications of 45 nt-long RNA molecules

Introduction RNA molecules have emerged as important molecules in therapeutics, primarily in the form of antisense oligos (ASO) and small interfering RNAs (siRNAs). Consequentially, requirements for...