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What is: Ion Exchange Chromatography (IEX) ?

Introduction Ion Exchange Chromatography (IEX) is a powerful and widely used technique for separating and purifying biomolecules such as proteins, peptides, and oligonucleotides. This technique is...
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QuickTip #5: Filter everything

Filtering is crucial in order to protect the packed column from all solid particles in feeds or eluents. Clogging of the column will increase back-pressure and disturb the feed loading zone causing...
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QuickTip #2: choosing IEX resin

Recovery of your biologically active target substance is a priority, and it’s important to check for its pH and ionic strength stability values...
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QuickTip #1: cleaning IEX columns

When an IEX resin has been used for some time, impurities may build up in the column causing loss of resolution or increase in back pressure (shown on the pressure monitor or seen by gap formation on...
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