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Addressing the challenge of optimizing His-tagged protein purifications

Introduction Immobilized Metal Affinity Chromatography (IMAC) is the commonest technique for purification of His-tagged proteins. Tagging proteins enables the use of affinity chromatography for...
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Easy optimization of His-tagged protein purification

Introduction Immobilized Metal Ion Affinity Chromatography (IMAC) utilizes the affinity of histidine, cysteine and tryptophan on the protein surface for binding to metal ions immobilized on the...
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