Ion Exchange Chromatography (IEX) is a powerful and widely used technique for separating and purifying biomolecules such as proteins, peptides, and oligonucleotides. This technique is based on the principle of electrostatic interactions between charged molecules and charged functional groups attached to a resin matrix. The technique offers a robust and highly selective method for separating molecules based on their charge, and is commonly employed in various biotechnological and pharmaceutical applications.
IEX can be divided into two main types: • Anion Exchange Chromatography – Targets negatively charged molecules which bind to positively charged resins. • Cation Exchange Chromatography – Targets positively charged molecules which bind to negatively charged resins.
The choice of resin type and functional group greatly influences the performance of the purification process.
Our IEX resins are specifically designed to offer optimal selectivity, resolution, and high dynamic binding capacity. This makes them ideal for purifying a wide range of biomolecules, including proteins, peptides, and oligonucleotides. WorkBeads resins provide excellent performance for peptides, including insulin, thanks to their high capacity and resolution, making them suitable for a broad range of peptide purification applications.
The technique is preferred in many laboratories and manufacturing facilities due to its: • High Purity and Yield: Effective removal of contaminants and impurities. • Scalability: Applicable from laboratory to industrial-scale operations. • Reproducibility: Consistent performance across multiple runs. • Versatility: Can be tailored to suit specific purification requirements.
We offer a variety of functional group alternatives to ensure compatibility with different biomolecules: • S (Strong Cation Exchange) – Sulfonic acid-based groups offering strong binding capacity for positively charged molecules. • Q (Strong Anion Exchange) – Quaternary amine groups designed for high-affinity binding of negatively charged molecules. • DEAE (Weak Anion Exchange) – Diethylaminoethyl groups providing mild interactions ideal for gentle purification of sensitive biomolecules. • TREN (Weak Cation Exchange) – Tris(2-aminoethyl)amine groups providing effective binding for positively charged molecules with improved selectivity.
Our WorkBeads series of resins are available with different functional groups, offering flexibility and high performance: • WorkBeads 40Q: Strong anion exchange for high-resolution separations. • WorkBeads 100Q: Optimized for high dynamic binding capacity and selectivity. • WorkBeads 40S: Strong cation exchange, providing excellent resolution. • WorkBeads 100S: Designed for maximum dynamic binding capacity for cation exchange processes. • WorkBeads 40 DEAE: A versatile resin for weak anion exchange chromatography. • WorkBeads 40 TREN: Ideal for weak cation exchange chromatography with enhanced selectivity.
We offer a range of prepacked columns to simplify and enhance your chromatography workflow: • GoBio Mini: Designed for small-scale purification and method optimization. • GoBio Screen: Perfect for screening conditions and preliminary separations. • GoBio Prep: Suitable for preparative purification with high loading capacity. • GoBio Prod: Tailored for larger-scale production processes. • GoBio Prod GMP: Specifically developed for Good Manufacturing Practice (GMP) environments, ensuring consistency and reliability.
IEX chromatography is extensively used in various industries including pharmaceuticals, biotechnology, and academic research. Its ability to provide high-purity separation of biomolecules makes it a preferred choice for: • Protein purification • Peptide separation • Oligonucleotide purification • Insulin and other large peptide purification • Enzyme purification • Antibody purification • Viral vector purification • Diagnostic reagent production
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