Applications

Virus and vaccine purification

WorkBeads SEC resins are an efficient and scalable approach for virus and vaccine purification that allows different buffers and pH to be easily used to achieve optimal separation.

Chromatography methods for virus and vaccine purification

Size exclusion chromatography is a separation technique that is very useful when purifying vaccines and viruses. The method is gentle towards the target molecules and different buffers and pH can easily be used. The vaccine or virus will pass through the column and elute earlier compared to smaller impurities.

Preparative virus purifications are often based on various filtration, precipitation, or ultracentrifugation techniques. None of these techniques are automated and they require time-consuming manual handling. There is a clear need for more efficient approaches for virus downstream processing. Chromatography is an alternative that offers automated and affordable purifications. WorkBeads SEC resins produce purified proteins at high yield and with high activity. 

WorkBeads give unbeatable results for virus and vaccine purification

High resolution separation

WorkBeads resins are optimized for high resolution separation of biomolecules at high flow rates to produce purified proteins at high yield.

High purity and yield

WorkBeads resins are designed using a proprietary method that ensures high purity and yield during the separation and purification of biomolecules.

Reproducible results

WorkBeads resins are available in pre-packed column formats, ensuring reproducible results from laboratory-scale purification to GMP manufacturing.

WorkBeads resins deliver high-yield purification of viruses and vaccines

WorkBeads SEC

WorkBeads SEC resins are used for preparative size exclusion chromatography for laboratory and large-scale purification of proteins, viruses, and other biomolecules by utilizing differences in their size. This resin is based on agarose, a biopolymer suitable for separation of biomolecules. WorkBeads resins are cross-linked using a proprietary method that results in a very rigid, highly porous, and physically stable matrix. Although the general recommendation for SEC is to use low flow rates for best purification, the rigidity and tight particle size distribution allows for the purification of viruses and other large substances at high flow rates for fast processing and high yields.

Size exclusion chromatography

Size exclusion chromatography (SEC) is a simple and reliable technique for the separation of molecular components according to their size. The technique relies on a separation resin of porous beads packed closely together in a column. The packed column is prepared by equilibration with a suitable running buffer, usually an aqueous buffer, before sample loading. SEC can provide efficient and scalable approaches for virus isolations compared to conventional methods, e.g., precipitation and sucrose density gradients. The most common approach in virus SEC purifications is to exploit the exclusion limit of the resins by eluting the macro-sized virus in the void volume while retaining the smaller impurities in the resin.

Download technical documents and examples

Poster

Purification of animal viral vaccine from cell supernatants by size exclusion chromatography

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Whitepaper

There is a clear need for more efficient approaches for virus downstream processing. Chromatography is an alternative that offers automated and affordable purifications. Here we present a virus purification setup that produces purified virus at high yield and with high activity.
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Application note

Size Exclusion Chromatography (SEC) is especially suited for biomolecules that are sensitive to, for example, pH, ionic strength, presence of metal ions, and temperature.  Choosing the most optimal resin for your target molecule not only depends on porosity or bead size but also on the application.
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Ensure purification success on your target molecule